VEGF-165 / VEGF-A (AA 27-191) (Active) Protein

Catalog No. ABIN7319413

This Recombinant VEGF-165 / VEGF-A protein is expressed in HEK-293 Cells.

Quick Overview for VEGF-165 / VEGF-A (AA 27-191) (Active) Protein (ABIN7319413)

Target: VEGF-165 / VEGF-A

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: ELISA

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 27-191

Purpose: Recombinant Human VEGF165/VEGFA Protein

Sequence: Ala27-Arg191

Characteristics: Recombinant Human VEGF165 is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.01 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized Human VEGF165 (PKSH033475) at 10μg/ml (100 μl/well) can bind Human VEGFR1-Fc (PKSH033445). The ED₅₀ of Recombinant Human VEGFR1-Fc (PKSH033445) is 36.4 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Citrate, 8 % Sucrose, 4 % Mannitol, 0.05 % Tween 80, pH 4.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: VEGF-165 / VEGF-A

Alternative Name: VEGF165/VEGFA

Background: MVCD1,VEGF,VEGF165,VPF,Vascular Endothelial Growth Factor Isoform 165,Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.

Molecular Weight:

Calculated MW: 19.1 kDa

Observed MW: 18-24 kDa

Gene ID: 7422

UniProt: P15692