Vaccinia Virus Soluble Interferon alpha/beta receptor B18 (VACV B18R) (AA 20-351) protein (His tag)

Catalog No. ABIN7321013

This Recombinant Vaccinia Virus Soluble Interferon alpha/beta receptor B18 protein is expressed in HEK-293 Cells.

Quick Overview for Vaccinia Virus Soluble Interferon alpha/beta receptor B18 (VACV B18R) (AA 20-351) protein (His tag) (ABIN7321013)

Target: Vaccinia Virus Soluble Interferon alpha/beta receptor B18 (VACV B18R)

Protein Type: Recombinant

Origin: Vaccinia Virus (VACV)

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 20-351

Purification tag / Conjugate: His tag

Purpose: Recombinant Vaccinia virus Soluble Interferon α/β Receptor B18 Protein (His Tag)

Sequence: His20-Glu351

Characteristics: Recombinant Vacciniavirus Soluble Interferon α / β receptor B18 is produced by our Mammalian expression system and the target gene encoding His20-Glu351 is expressed with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Vaccinia Virus Soluble Interferon alpha/beta receptor B18 (VACV B18R)

Alternative Name: Soluble Interferon alpha/beta receptor B18

Background: Soluble interferon alpha/beta receptor B18,VACWR200,B18R, also known as B19R in the Copenhagen strain of Vaccinia, is a type I interferon (IFN)-binding protein encoded by the B18R open reading frame in the Western Reserve strain of vaccinia virus. B18R exists in two forms, soluble and membrane-bound form and also has a broad species specificity. B18R has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta. Secreted B18R binds to uninfected and infected cells. B18R presents at the cell surface and protects cells from the antiviral state. It has shown that binding of soluble recombinant B18R protects cultured cells from IFN and allows vaccinia virus replication.

Molecular Weight:

Calculated MW: 39.2 kDa

Observed MW: 50-65 kDa

UniProt: P25213