ZIKV E Protein (AA 291-399, AA 426-485) (His-MBP)

Catalog No. ABIN7198748

Recombinant ZIKV E protein expressed in Escherichia coli (E. coli).

Quick Overview for ZIKV E Protein (AA 291-399, AA 426-485) (His-MBP) (ABIN7198748)

Target: ZIKV E (Zika Virus Envelope (ZIKV E))

Protein Type: Recombinant

Origin: Zika Virus (ZIKV)

Source: Escherichia coli (E. coli)

Purity: > 75 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 291-399, AA 426-485

Purification tag / Conjugate: This ZIKV E protein is labelled with His-MBP.

Purpose: Recombinant ZIKV E / Envelope protein (Domain I, His & MBP Tag)

Sequence: Ile291-Thr399 and Glu426-Gly485 and Ser576- Gly592

Characteristics: A DNA sequence encoding the Zika virus (strain Zika SPH2015) E (ALU33341.1) (Ile291-Thr399 and Glu426-Gly485 and Ser576- Gly592) was expressed with a N-terminal polyhistidine-tagged MBP tag at the N-terminus (his-MBP).

Sterility: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 250 mM NaCl, 50 mM Tris, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: ZIKV E (Zika Virus Envelope (ZIKV E))

Alternative Name: Zika Envelope

Background: E protein,Zika virus,strain Zika SPH2015,Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF.Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76 % of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.

Molecular Weight: 57.2 kDa