DDR2 Protein (His tag)

Catalog No. ABIN7320240

This Recombinant DDR2 protein is expressed in HEK-293 Cells.

Quick Overview for DDR2 Protein (His tag) (ABIN7320240)

Target: DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This DDR2 protein is labelled with His tag.

Purpose: Recombinant Mouse DDR2 Kinase/CD167b Protein (His Tag)(Active)

Sequence: Met 1-Arg 399

Characteristics: A DNA sequence encoding the extracellular domain of mouse DDR2 (NP_072075.2) (Met 1-Arg 399) was expressed, with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to bind rat tail Collagen I in a functional ELISA.

Application Details

Comment:

80-90 kDa

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))

Alternative Name: DDR2

Background:

Background: Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: Discoidin domain-containing receptor 2; Discoidin domain receptor 2; CD167 antigen-like family member B; Neurotrophic tyrosine kinase; receptor-related 3; Receptor protein-tyrosine kinase TKT; Tyrosine-protein kinase TYRO10; CD167b; Ddr2;Ntrkr3;tyro10

Molecular Weight: 44 kDa

NCBI Accession: NP_072075

Pathways: RTK Signaling