MERS-CoV Nucleocapsid Protein (His tag)

Catalog No. ABIN7198809

This Recombinant MERS-CoV Nucleocapsid protein is produced in Baculovirus infected Insect Cells.

Quick Overview for MERS-CoV Nucleocapsid Protein (His tag) (ABIN7198809)

Target: MERS-CoV Nucleocapsid (MERS-CoV N) (MERS-Coronavirus Nucleocapsid Protein (MERS-CoV N))

Protein Type: Recombinant

Origin: Middle East Respiratory Syndrome Coronavirus (MERS-CoV)

Source: Baculovirus infected Insect Cells

Purity: > 90 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This MERS-CoV Nucleocapsid protein is labelled with His tag.

Purpose: Recombinant MERS-CoV Nucleoprotein / NP protein (His Tag)

Sequence: Met1-Asp413

Characteristics: A DNA sequence encoding the Nucleoprotein MERS-CoV (AFS88943.1) (Met1-Asp413) was fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % gly, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: MERS-CoV Nucleocapsid (MERS-CoV N) (MERS-Coronavirus Nucleocapsid Protein (MERS-CoV N))

Alternative Name: MERS-Coronavirus Nucleocapsid Protein

Background:

Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Synonym: coronavirus NP Protein, MERS-CoV, coronavirus Nucleocapsid Protein, MERS-CoV, coronavirus Nucleoprotein Protein, MERS-CoV, cov np Protein, MERS-CoV, ncov NP Protein, MERS-CoV, novel coronavirus Nucleoprotein Protein, MERS-CoV, NP Protein, MERS-CoV, Nucleocapsid Protein, MERS-CoV, Nucleoprotein Protein, MERS-CoV

Molecular Weight: 46.51kDa.