BTK Kinase (Active) protein (His tag)

Catalog No. ABIN7317103

This Recombinant BTK Kinase protein is produced in Baculovirus infected Insect Cells.

Quick Overview for BTK Kinase (Active) protein (His tag) (ABIN7317103)

Target: BTK Kinase

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 85 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant Human Bruton Tyrosine Kinase/BTK Kinase Protein (His Tag)(Active)

Sequence: Met 1-Ser 659

Characteristics: A DNA sequence encoding the human BTK (NP_000052.1) (Met 1-Ser 659) was expressed with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: 1. The specific activity was determined to be 115 nmol/min/mg using Poly(Glu,Tyr)4:1 peptide as substrate.2. Measured by its ability to bind human BLNK in a functional ELISA.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.0

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: BTK Kinase

Background:

Background: Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: AGMX1;AT;ATK;BPK;IMD1;PSCTK1;XLA

Molecular Weight: 77.8 kDa

NCBI Accession: NP_000052