Calcineurin B Protein (CAN) (His tag)

Catalog No. ABIN7318221

This Recombinant Calcineurin B protein is expressed in Escherichia coli (E. coli).

Quick Overview for Calcineurin B Protein (CAN) (His tag) (ABIN7318221)

Target: Calcineurin B (CAN)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Calcineurin B protein is labelled with His tag.

Purpose: Recombinant Human Calcineurin B/CNB Protein (His Tag)

Sequence: Met 1-Val170

Characteristics: Recombinant Human Calcineurin Subunit B Type 1 is produced by our E.coli expression system and the target gene encoding Met1-Val170 is expressed with a 6His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 2 mM DTT, pH 8.0 .
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Calcineurin B (CAN)

Alternative Name: Calcineurin B

Background: CNA2,CNB,Calcineurin Subunit B Type 1,PPP3R1,Protein Phosphatase 2B Regulatory Subunit 1,Protein Phosphatase 3 Regulatory Subunit B Alpha Osoform 1,Calcineurin Subunit B Type 1 belongs to the calcineurin regulatory subunit family. Calcineurin Subunit B Type 1 is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase. It is composed of a catalytic subunit (A) and a regulatory subunit (B). It contains four EF-hand domains and four functional calcium-binding sites. Calcineurin Subunit B Type 1 plays an improtant role in the T cell activation pathway.

Molecular Weight:

Calculated MW: 21.5 kDa

Observed MW: 18-22 kDa

Gene ID: 5534

UniProt: P63098

Pathways: Cellular Glucan Metabolic Process, VEGF Signaling