CAMK1 Protein

Catalog No. ABIN7317143

This Recombinant CAMK1 protein is produced in Escherichia coli (E. coli).

Quick Overview for CAMK1 Protein (ABIN7317143)

Target: CAMK1 (Calcium/calmodulin-Dependent Protein Kinase I (CAMK1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 85 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human CAMK1/CaMKI-alpha Protein

Sequence: Leu 2-Leu 370

Characteristics: A DNA sequence encoding the human CAMK1 (NP_003647.1) (Leu 2-Leu 370) was expressed and purified, with two additional amino acids (Gly & Pro) at the N-terminus.

Sterility: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tirs, 150 mM NaCl, 10 % glycerol, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CAMK1 (Calcium/calmodulin-Dependent Protein Kinase I (CAMK1))

Alternative Name: CAMK1

Background: CAMK1,CaM Kinase I,CaM Kinase I Alpha,CaM-KI,CaMKI-Alpha,Calcium/Calmodulin-Dependent Protein Kinase Type 1,Calcium/calmodulin-dependent protein kinase orCaM kinases are serine/threonine-specific protein kinasesthat are primarily regulated by the Calcium/calmodulin complex. These kinases show a memory effect on activation. CaM kinases activity can outlast the intracellular calcium transient that is needed to activate it. Inneurons, this property is important for the induction of synaptic plasticity. Pharmacological inhibition of CaM kinases II blocks the induction oflong-term potentiation. Upon activation, CaM kinases II phosphorylates postsynaptic glutamate receptors and changes the electrical properties of the synapse. Calcium/calmodulin-dependent protein kinase type 1D, also known as CaM kinase I delta, CaM kinase ID, CaMKI-like protein kinase, CKLiK and CAMK1D, is a member of theprotein kinase superfamily and CaMK subfamily. It contains oneprotein kinase domain. CAMK1D is broadly expressed. It is highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. Engineered overexpression of CAMK1D in non-tumorigenic breast epithelial cells led to increased cell proliferation, and molecular and phenotypic alterations indicative of epithelial-mesenchymal transition (EMT), including loss of cell-cell adhesions and increased cell migration and invasion. CAMK1D is a potential therapeutic target with particular relevance to clinically unfavorable basal-like tumors.

Molecular Weight:

Calculated MW: 41.5 kDa

Observed MW: 42 kDa

Gene ID: 8536

NCBI Accession: NP_003647

UniProt: Q14012

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Smooth Muscle Cell Migration