HLA-B*27:05 protein (SBP Tag)

Catalog No. ABIN7273193

Recombinant HLA-B*27:05 protein expressed in Mammalian Cells.

Quick Overview for HLA-B*27:05 protein (SBP Tag) (ABIN7273193)

Target: HLA-B*27:05

Protein Type: Recombinant

Origin: Human

Source: Mammalian Cells

Application: ELISA

Purity: > 80 %

Product Details

Purification tag / Conjugate: SBP Tag

Purpose: The product is HLA-B*27:05 complexed with β2-microglobulin (b2m)

Sequence: heavy chain: GSHSMRYFHT SVSRPGRGEP RFITVGYVDD TLFVRFDSDA ASPREEPRAP WIEQEGPEYW DRETQICKAK AQTDREDLRT LLRYYNQSEA GSHTLQNMYG CDVGPDGRLL RGYHQDAYDG KDYIALNEDL SSWTAADTAA QITQRKWEAA RVAEQLRAYL EGECVEWLRR YLENGKETLQ RADPPKTHVT HHPISDHEAT LRCWALGFYP AEITLTWQRD GEDQTQDTEL VETRPAGDRT FQKWAAVVVP SGEEQRYTCH VQHEGLPKPL TLRWEPSSQS TVPI light chain: IQRTPKIQVY SRHPAENGKS NFLNCYVSGF HPSDIEVDLL KNGERIEKVE HSDLSFSKDW SFYLLYYTEF TPTEKDEYAC RVNHVTLSQP KIVKWDRDM Sequences without tags. Tag location is at the discretion of the manufactur er. If you have a special request, please contact us.

Specificity: HLA-B*27:05 complexed with b2m, P01889 / P61769
HLA-B*27:05 is associated with a higher incidence of ankylosing spondylitis in Caucasians and is therefore of great interest to HLA researchers. HLA-B*27:05 is recognized by anti-Bw4 antibodies.

Characteristics: HLAs are produced in mammalian cell cultures as recombinant, truncated, soluble antigens, in complex with β2-microglobulin (HLA class I). As part of our quality control, all HLA are analyzed for purity by gel electrophoresis, concentration is determined by ELISA (0.5 mg/mL) and their functionality is verified with a specific antiserum.
contains endogenous peptides.

Purification: Chromatography

Application Details

Application Notes: ELISA: 1 - 100 ng/mL

Comment:

reactive with antibodies W6,32,BBM.1,specific antiserum, B cell ELI Spot (predicted), in vitro memory B cell stimulation (predicted), HLA competition assay (predicted), control in various assays (predicted)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: TRIS based buffer, pH 8.0, Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shelf life: 6 month from date of production. Store at 2 °C - 8 °C

Expiry Date: 6 months

Target Details

Target: HLA-B*27:05

Background: Human Leukozyte Antigen, human Antigen-presenting major histocompatibility complex class I (MHCI) molecule. Expressed on all cells, displays peptides to CD8-positive T cells. Involved in cellular immune response.

Molecular Weight: approx. 50 kDa (38 kDa heavy chain + 12 kDa b2m)

UniProt: P01889