ACE2 Protein (AA 18-740) (Biotin,His-Avi Tag)

Catalog No. ABIN7273794

This Recombinant ACE2 protein is produced in HEK-293 Cells.

Quick Overview for ACE2 Protein (AA 18-740) (Biotin,His-Avi Tag) (ABIN7273794)

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 18-740

Purification tag / Conjugate: This ACE2 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human ACE2/ACEH Protein

Sequence: Gln18-Ser740

Characteristics: Recombinant Biotinylated Human ACE2/ACEH Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln18-Ser740.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized SARS-COV-2 Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 40.6ng/ml determined by ELISA (QC Test).,Biotinylated Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded SARS-COV-2 Spike S1, hFc Tag on ProA-Biosensor can bind Biotinylated Human ACE2 with an affinity constant of 2.01 nM as determined in BLI assay (Gator® Prime).,The purity of Biotinylated human ACE2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: ACE2

Background: ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.

Molecular Weight: 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Bis-Tris PAGE result.

UniProt: Q9BYF1

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour