Amphiregulin Protein (AREG) (AA 101-187) (Fc Tag)
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- Target See all Amphiregulin (AREG) Proteins
- Amphiregulin (AREG)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 101-187
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Amphiregulin protein is labelled with Fc Tag.
- Purpose
- Human AREG Protein
- Sequence
- Ser101-Lys187
- Characteristics
- Recombinant Human AREG Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ser101-Lys187.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human AREG, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product AREG Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Amphiregulin (AREG)
- Alternative Name
- AREG (AREG Products)
- Synonyms
- AR Protein, CRDGF Protein, SDGF Protein, Sdgf Protein, AREG Protein, AREGB Protein, AREG/SDGF Protein, amphiregulin Protein, AREG Protein, Areg Protein
- Background
- Amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and is expressed in a plethora of cancers. Tumour growth and metastasis were decreased by AREG silencing in an orthotopic model of pancreatic cancer. AREG may play a critical role in cell migration, invasion, and EMT by activating the EGFR/ERK/NF-κB signalling pathway in pancreatic cancer cells.
- Molecular Weight
- 37.3 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
- UniProt
- P15514
- Pathways
- RTK Signaling, EGFR Signaling Pathway
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