Amphiregulin Protein (AREG) (AA 101-187) (hFc Tag)

Catalog No. ABIN7273804

This Recombinant Amphiregulin protein is expressed in HEK-293 Cells.

Quick Overview for Amphiregulin Protein (AREG) (AA 101-187) (hFc Tag) (ABIN7273804)

Target: Amphiregulin (AREG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 101-187

Purification tag / Conjugate: This Amphiregulin protein is labelled with hFc Tag.

Purpose: Human AREG Protein

Sequence: Ser101-Lys187

Characteristics: Recombinant Human AREG Protein is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.It contains Ser101-Lys187.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human AREG, hFc Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 32.5ng/ml determined by ELISA (QC Test).,Human-AREG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human-AREG is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: Amphiregulin (AREG)

Alternative Name: AREG

Background: Amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and is expressed in a plethora of cancers. Tumour growth and metastasis were decreased by AREG silencing in an orthotopic model of pancreatic cancer. AREG may play a critical role in cell migration, invasion, and EMT by activating the EGFR/ERK/NF-κB signalling pathway in pancreatic cancer cells.

Molecular Weight: 37.3 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.

UniProt: P15514

Pathways: RTK Signaling, EGFR Signaling Pathway