CD166 Protein (AA 28-527) (Biotin,His-Avi Tag)

Catalog No. ABIN7273820

Recombinant CD166 protein expressed in HEK-293 Cells.

Quick Overview for CD166 Protein (AA 28-527) (Biotin,His-Avi Tag) (ABIN7273820)

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 28-527

Purification tag / Conjugate: This CD166 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Mouse ALCAM/CD166 Protein

Sequence: Tyr28-Lys527

Characteristics: Recombinant Biotinylated Mouse ALCAM/CD166 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr28-Lys527.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Immobilized Human CD6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ALCAM, His-Avi Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test).,Biotinylated Mouse ALCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Mouse ALCAM is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Alternative Name: ALCAM

Background: Activated Leukocyte Cell Adhesion Molecule (ALCAM/CD166) is a glycoprotein involved in homotypic and heterotypic cell adhesion. ALCAM can be proteolytically cleaved at the cell surface by metalloproteases, which generate shedding of its ectodomain. In various tumors, ALCAM is overexpressed and serves as a valuable prognostic marker of disease progression.

Molecular Weight: 58.9 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Bis-Tris PAGE result.

UniProt: Q61490