Annexin A2 Protein (ANXA2) (AA 2-339) (His tag)

Catalog No. ABIN7273846

This Recombinant Annexin A2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Annexin A2 Protein (ANXA2) (AA 2-339) (His tag) (ABIN7273846)

Target: Annexin A2 (ANXA2)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 2-339

Purification tag / Conjugate: This Annexin A2 protein is labelled with His tag.

Purpose: Mouse ANXA2 Protein

Sequence: Ser2-Asp339

Characteristics: Recombinant Mouse ANXA2 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ser2-Asp339.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse ANXA2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 20 mM MES, 150 mM NaCl, 200 mM L-arginine, 5 mM DTT ( pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM MES, 150 mM NaCl, 200 mM L-arginine, 5 mM DTT ( pH 5.5). Normally 8 % trehalose is added as protectant before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Annexin A2 (ANXA2)

Alternative Name: ANXA2

Background: ANXA2, highly expressed in invasive breast cancer cells, is closely related with poor prognosis, and acts as a molecular switch to EGFR activation. ANXA2 expression is inversely correlated with cell sensitivity to gefitinib. Knockdown of ANXA2 expression in MDA-MB-231 cells increased the gefitinib induced cell death. When ANXA2 was overexpressed in MCF7 cells, the gefitinib induced cell death was decreased. Furthermore, the phosphorylation of ANXA2 at Tyr23 is negatively correlated with the sensitivity of TNBC to gefitinib.

Molecular Weight: 39.6 kDa same as Bis-Tris PAGE result.

UniProt: P07356

Pathways: S100 Proteins