ApoE3 Protein (AA 19-317) (His tag,Biotin)
ApoE3
Origin: Human
Host: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE
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- Target See all ApoE3 products
- ApoE3 (Apolipoprotein E3 (ApoE3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 19-317
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ApoE3 protein is labelled with His tag,Biotin.
- Purpose
- Biotinylated Human APOE3/Apolipoprotein E Protein (Primary Amine Labeling)
- Sequence
- Lys19-His317
- Characteristics
- Recombinant Biotinylated Human APOE3/Apolipoprotein E Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus.It contains Lys19-His317.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 1.41 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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Apolipoprotein E3 (ApoE3) (AA 19-317) protein (His tag)
ApoE3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- ApoE3 (Apolipoprotein E3 (ApoE3))
- Alternative Name
- APOE3 (ApoE3 Products)
- Synonyms
- AD2 Protein, LDLCQ5 Protein, LPG Protein, apolipoprotein E Protein, APOE Protein
- Background
- Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).
- Molecular Weight
- 35.3 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
- Pathways
- Regulation of Cell Size, Lipid Metabolism
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