AXL Protein (AA 26-449) (His-Avi Tag)

Catalog No. ABIN7273884

This Recombinant AXL protein is produced in HEK-293 Cells.

Quick Overview for AXL Protein (AA 26-449) (His-Avi Tag) (ABIN7273884)

Target: AXL (AXL Receptor tyrosine Kinase (AXL))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-449

Purification tag / Conjugate: This AXL protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human AXL Protein

Sequence: Ala26-Pro449

Characteristics: Recombinant Human AXL Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Pro449.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human AXL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.,Human AXL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-Axl Ab., hFc Tag on ProA-Biosensor can bind Human Axl, His Tag with an affinity constant of 0.14 μM as determined in BLI assay (Gator® Prime).,The purity of Human AXL is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: AXL (AXL Receptor tyrosine Kinase (AXL))

Alternative Name: AXL

Background: Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.

Molecular Weight: 48.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

UniProt: P30530

Pathways: RTK Signaling, Cellular Response to Molecule of Bacterial Origin