CD86 Protein (CD86) (AA 26-247) (His-Avi Tag,Biotin)

Catalog No. ABIN7273899

Product Details

Target: CD86

Protein Type: Recombinant

Protein Characteristics: AA 26-247

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD86 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human B7-2/CD86 Protein

Sequence: Leu26-Pro247

Characteristics: Recombinant Biotinylated Human B7-2/CD86 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu26-Pro247.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human B7-2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.2μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD86

Alternative Name: B7-2 (CD86 Products)

Background: B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.

Molecular Weight: 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

UniProt: P42081-1

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation