CD86 Protein (CD86) (AA 26-247) (Fc Tag)
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- Target See all CD86 Proteins
- CD86
- Protein Type
- Recombinant
- Protein Characteristics
- AA 26-247
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD86 protein is labelled with Fc Tag.
- Purpose
- Human B7-2/CD86 Protein
- Sequence
- Leu26-Pro247
- Characteristics
- Recombinant Human B7-2/CD86 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu26-Pro247.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CTLA-4, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human B7-2, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD86
- Alternative Name
- B7-2 (CD86 Products)
- Synonyms
- B7-2 Protein, B7.2 Protein, B70 Protein, CD28LG2 Protein, LAB72 Protein, B7 Protein, CLS1 Protein, Cd28l2 Protein, ETC-1 Protein, Ly-58 Protein, Ly58 Protein, MB7 Protein, MB7-2 Protein, TS/A-2 Protein, CD86 molecule Protein, CD86 antigen Protein, integrin subunit alpha 2b Protein, CD86 Protein, Cd86 Protein, Itga2b Protein
- Background
- B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
- Molecular Weight
- 52 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Tris-Bis PAGE result.
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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