TNFSF9 Protein (AA 71-254, Trimer) (Fc Tag,DYKDDDDK Tag)

Catalog No. ABIN7273929

This Recombinant TNFSF9 protein is produced in HEK-293 Cells.

Quick Overview for TNFSF9 Protein (AA 71-254, Trimer) (Fc Tag,DYKDDDDK Tag) (ABIN7273929)

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 71-254, Trimer

Purification tag / Conjugate: This TNFSF9 protein is labelled with Fc Tag,DYKDDDDK Tag.

Purpose: Human 4-1BB Ligand/TNFSF9 Trimer Protein

Sequence: Arg71-Glu254

Characteristics: Recombinant Human 4-1BB Ligand/TNFSF9 Trimer Protein is expressed from HEK293 with Flag tag and monomeric hFc tag at the N-Terminus.It contains Arg71-Glu254 trimer design.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human 4-1BB, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).,Human 4-1BB Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human 4-1BB Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Alternative Name: 4-1BB Ligand

Background: The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.

Molecular Weight: 84.3 kDa same as Bis-Tris PAGE result.

UniProt: P41273

Pathways: Activated T Cell Proliferation, Cancer Immune Checkpoints