B7-H3 (4Ig) (AA 27-461) protein (His-Avi Tag)
Quick Overview for B7-H3 (4Ig) (AA 27-461) protein (His-Avi Tag) (ABIN7273965)
Target
Protein Type
Origin
Source
Purity
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Protein Characteristics
- AA 27-461
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Purification tag / Conjugate
- His-Avi Tag
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Purpose
- Human B7-H3 (4Ig) /B7-H3b Protein
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Sequence
- Gly27-Thr461
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Characteristics
- Recombinant Human B7-H3 (4Ig)/B7-H3b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly27-Thr461.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human B7-H3 (4Ig) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA. See testing image for detail.
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B7-H3 (4Ig) (AA 27-461) (Active) protein (Biotin,His-Avi Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
B7-H3 (4Ig) (AA 27-461) (Active) protein (His tag)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
B7-H3 (4Ig) (AA 27-461) (Active) protein (Fc Tag,AVI tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
B7-H3 (4Ig) (AA 27-461) (Active) protein (His tag,AVI tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
B7-H3 (4Ig) (AA 27-461) (Active) protein (Fc Tag)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
B7-H3 (4Ig) (AA 27-461) (Active) protein (His tag,FITC)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- B7-H3 (4Ig)
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Background
- B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig-B7-H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
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Molecular Weight
- 49.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
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UniProt
- Q5ZPR3-1
Target
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