CD276 Protein (CD276) (AA 29-245) (Biotin,His-Avi Tag)

Catalog No. ABIN7273969

This Recombinant CD276 protein is expressed in HEK-293 Cells.

Quick Overview for CD276 Protein (CD276) (AA 29-245) (Biotin,His-Avi Tag) (ABIN7273969)

Target: CD276

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 29-245

Purification tag / Conjugate: This CD276 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human B7-H3/CD276 Protein

Sequence: Leu29-Pro245

Characteristics: Recombinant Biotinylated Human B7-H3/CD276 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu29-Pro245.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human B7-H3, His Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 2.8 ng/ml determined by ELISA.,Biotinylated Human B7-H3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human B7-H3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt., -80°C for 3 months after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: CD276

Alternative Name: B7-H3

Background: B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers. B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig-B7-H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.

Molecular Weight: 26.49 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

UniProt: Q5ZPR3

Pathways: Cancer Immune Checkpoints