BST1 Protein (AA 25-286) (His tag)

Catalog No. ABIN7273994

Recombinant BST1 protein expressed in HEK-293 Cells.

Quick Overview for BST1 Protein (AA 25-286) (His tag) (ABIN7273994)

Target: BST1 (Bone Marrow Stromal Cell Antigen 1 (BST1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-286

Purification tag / Conjugate: This BST1 protein is labelled with His tag.

Purpose: Mouse BST1 Protein

Sequence: Ala25-Ser286

Characteristics: Recombinant Mouse BST1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala25-Ser286.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse BST1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse BST1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: BST1 (Bone Marrow Stromal Cell Antigen 1 (BST1))

Alternative Name: BST1

Background: BST1 overexpression conferred resistance to sphingosine in yeast. BST1 deletion produced sensitivity to exogenous D-erythro-sphingosine and phytosphingosine and intracellular accumulation of sphingosine 1-phosphate upon exposure to exogenous sphingosine. sphingoid base metabolism is similar in all eukaryotes and suggests that yeast genetics may be useful in the isolation and identification of other genes involved in sphingolipid signaling and metabolism.

Molecular Weight: 30.7 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.

UniProt: Q64277