PTK7 Protein (AA 31-704) (His tag)

Catalog No. ABIN7274040

This Recombinant PTK7 protein is produced in HEK-293 Cells.

Quick Overview for PTK7 Protein (AA 31-704) (His tag) (ABIN7274040)

Target: PTK7 (PTK7 Protein tyrosine Kinase 7 (PTK7))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-704

Purification tag / Conjugate: This PTK7 protein is labelled with His tag.

Purpose: Human PTK7/CCK4 Protein

Sequence: Ala31-Thr704

Characteristics: Recombinant Human PTK7/CCK4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala31-Thr704.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human PTK7, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PTK7 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.,Human PTK7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human PTK7 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PTK7 (PTK7 Protein tyrosine Kinase 7 (PTK7))

Alternative Name: PTK7

Background: Protein Tyrosine Kinase 7 (PTK7) is as a critical regulator of canonical and non-canonical Wnt-signaling during embryonic development and cancer cell formation. Disrupting PTK7 activity perturbs vertebrate nervous system development, and also promotes human cancer formation. Observations in different model systems suggest a complex cross-talk between PTK7 protein and Wnt signaling.

Molecular Weight: 75.7 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Bis-Tris PAGE result.

UniProt: Q13308

Pathways: RTK Signaling, Tube Formation