CD177 Protein (AA 26-516) (His tag)

Catalog No. ABIN7274065

This Recombinant CD177 protein is expressed in HEK-293 Cells.

Quick Overview for CD177 Protein (AA 26-516) (His tag) (ABIN7274065)

Target: CD177 (CD177 Molecule (CD177))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-516

Purification tag / Conjugate: This CD177 protein is labelled with His tag.

Purpose: Human CD117 Protein

Sequence: Gln26-Thr516

Characteristics: Recombinant Human CD117 Protein is expressed from HEK293 with His tag at the C-Terminus., It contains Gln26-Thr516.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.73ug/ml determined by ELISA.,Immobilized Human CD117, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA.,Human CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-CD117 Ab., hFc Tag on ProA-Biosensor can bind Human CD117, His Tag with an affinity constant of 6.08 nM as determined in BLI assay (Gator® Prime).,The purity of Human CD117 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD177 (CD177 Molecule (CD177))

Alternative Name: CD177

Background: The stem cell factor receptor (SCFR, CD117) is encoded by the c-kit proto-oncogene, which is the cellular counterpart of the v-kit oncogene of a transforming feline retrovirus (HZ4-feline sarcoma virus). It is a 145kD transmembrane glycoprotein which is homologous to several other growth factor receptors such as the receptors for macrophage colony stimulating factor (M-CSF, CSF-1, c-fms), platelet-derived growth factor a and b (PDGF-Ra , PDGF-Rb ), and the newly described growth factor receptor STK-1, which is the human homologue of the murine FLK2/FLT3 receptor.

Molecular Weight: 56.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result.

UniProt: P10721