Poliovirus Receptor Protein (PVR) (AA 21-343) (Biotin,His-Avi Tag)

Catalog No. ABIN7274081

Recombinant Poliovirus Receptor protein expressed in HEK-293 Cells.

Quick Overview for Poliovirus Receptor Protein (PVR) (AA 21-343) (Biotin,His-Avi Tag) (ABIN7274081)

Target: Poliovirus Receptor (PVR)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-343

Purification tag / Conjugate: This Poliovirus Receptor protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human CD155/PVR Protein

Sequence: Trp21-Asn343

Characteristics: Recombinant Biotinylated Human CD155/PVR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp21-Asn343.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human CD155, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).,Immobilized Biotinylated Human CD155, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA.,Biotinylated Human CD155 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Biotinylated Human CD155 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Poliovirus Receptor (PVR)

Alternative Name: CD155

Background: CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.

Molecular Weight: 38 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

UniProt: P15151

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interactome