Poliovirus Receptor Protein (PVR) (AA 29-348) (His tag)

Catalog No. ABIN7274094

This Recombinant Poliovirus Receptor protein is produced in HEK-293 Cells.

Quick Overview for Poliovirus Receptor Protein (PVR) (AA 29-348) (His tag) (ABIN7274094)

Target: Poliovirus Receptor (PVR)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 29-348

Purification tag / Conjugate: This Poliovirus Receptor protein is labelled with His tag.

Purpose: Mouse CD155/PVR Protein

Sequence: Asp29-Leu348

Characteristics: Recombinant Mouse CD155/PVR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp29-Leu348.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse CD155, His Tag immobilized on CM5 Chip can bind Mouse TIGIT, His Tag with an affinity constant of 4.00 uM as determined in SPR assay (Biacore T200).,Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA (QC Test).,Mouse CD155 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Mouse CD155, His Tag on Anti-His-Biosensor can bind Rat TIGIT, mFc Tag with an affinity constant of 6.93 nM as determined in BLI assay (Gator® Prime).,The purity of Mouse CD155 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Poliovirus Receptor (PVR)

Alternative Name: CD155

Background: CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.

Molecular Weight: 35.9 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Bis-Tris PAGE result.

UniProt: Q8K094

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interactome