CD21 Protein (AA 21-971) (His tag)

Catalog No. ABIN7274107

This Recombinant CD21 protein is expressed in HEK-293 Cells.

Quick Overview for CD21 Protein (AA 21-971) (His tag) (ABIN7274107)

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-971

Purification tag / Conjugate: This CD21 protein is labelled with His tag.

Purpose: Human CD21 Protein

Sequence: Ile21-Arg971

Characteristics: Recombinant Human CD21 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile21-Arg971.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD21, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD21 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.,Human CD21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD21 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Alternative Name: CD21

Background: CD21 mRNA expression, an EBV-entry receptor, was transiently detected in NK cells after exosome treatment, and levels decreased after further culture. CD21 protein expression was also transiently transferred to NK cells after co-culture with an EBV-positive Burkitt lymphoma cell line (Raji) via trogocytosis. However, EBV did not infect NK cells through CD21-mediated trogocytosis.

Molecular Weight: 105.3 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.

UniProt: P20023

Pathways: Complement System