CD21 Protein (His tag)

Catalog No. ABIN7317757

Product Details

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD21 protein is labelled with His tag.

Purpose: Recombinant Human CD21/CR2/C3DR Protein (His Tag)(Active)

Sequence: Met 1-Arg 971

Characteristics: A DNA sequence encoding the human CD21 isoform A (P20023-1) extracellular domain (Met 1-Arg 971) was expressed, with a carboxy-terminal polyhistidine tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized human CD21 at 2 μg/ml (100 μl/well) can bind biotinylated human CD9. The EC50 of biotinylated human CD9 is 1.76 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Alternative Name: CD21/CR2/C3DR (CR2 Products)

Synonyms: C3DR Protein, CD21 Protein, CR Protein, CVID7 Protein, SLEB9 Protein, Cd21 Protein, cd21 Protein, CR1 Protein, CR1-L Protein, CR2 Protein, complement C3d receptor 2 Protein, complement C3b/C4b receptor 1 (Knops blood group) Protein, CR2 Protein, Cr2 Protein, CR1 Protein

Background:

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD21, also known as Complement component (3d / Epstein Barr virus) receptor 2 and CR2, is a member of the CD system and is a protein involved in complement system. CD21 is present on all mature B-cells and some T-cells and follicular dendritic cells. CD21 on mature B-cells form a complex called the B cell receptor complex with two other membrane proteins, CD19 and CD81. CD21 has a function in the complement system through serving as the cellular receper specific for ligands such as C3 and C4 which can be attached to foreign macromolecules in order to remove or uptake them. This results in B-cells having enhanced response to the antigen.

Synonym: C3DR,CD21,CR,CVID7,SLEB9

Molecular Weight: 106 kDa

Pathways: Complement System