CD21 Protein (AA 12-963) (His tag)

Catalog No. ABIN7274121

This Recombinant CD21 protein is produced in HEK-293 Cells.

Quick Overview for CD21 Protein (AA 12-963) (His tag) (ABIN7274121)

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 12-963

Purification tag / Conjugate: This CD21 protein is labelled with His tag.

Purpose: Mouse CD21 Protein

Sequence: Ile12-Trp963

Characteristics: Recombinant Mouse CD21 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile12-Trp963.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse CD21 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Alternative Name: CD21

Background: A natural soluble form of CD21 that is cleaved from lymphocyte membrane CD21 circulates in normal human serum. Soluble CD21 retains the capacity to bind iC3b and CD23, the known ligands of membrane CD21. In a similar fashion to IgE complexes, another ligand of CD23, the soluble CD21 was shown to efficiently trigger CD23-signalling pathways in human monocytes.

Molecular Weight: 106.03 kDa. Due to glycosylation, the protein migrates to 130-160 kDa based on Bis-Tris PAGE result.

UniProt: P19070

Pathways: Complement System