CD3E & CD3G (AA 23-126) (Active) protein (Biotin,His tag)

Catalog No. ABIN7274145

This Recombinant CD3E & CD3G protein is produced in HEK-293 Cells.

Quick Overview for CD3E & CD3G (AA 23-126) (Active) protein (Biotin,His tag) (ABIN7274145)

Target: CD3E & CD3G

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-126

Purification tag / Conjugate: Biotin,His tag

Purpose: Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (Primary Amine Labeling)

Sequence: Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G)

Specificity: Uni-Prot: P07766 (CD3E), P09693 (CD3G)

Characteristics: Recombinant Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus of CD3G.It contains Asp23-Asp126 (CD3E) and Gln23-Ser116 (CD3G).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human CD3E&CD3G, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD3 Antibody, mFc Tag with the EC50 of 64.5ng/ml determined by ELISA (QC Test).,Biotinylated Human CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD3E & CD3G

Alternative Name: CD3E&CD3G

Background: T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Molecular Weight: 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.

UniProt: P07766