CD3E & CD3G (AA 23-126) (Active) protein (His tag,Fc Tag,DYKDDDDK Tag)

Catalog No. ABIN4949087

Product Details

Target: CD3E & CD3G

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 23-126

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: His tag,Fc Tag,DYKDDDDK Tag

Application: Functional Studies (Func)

Sequence: AA 23-126

Characteristics: The heterodimer Human CD3 epsilon & CD3 gamma is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: CD3E & CD3G

Background: T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Molecular Weight: 39.2 kDa (CD3E) & 38.6 kDa (CD3G)