TNFSF8 Protein (AA 63-234) (mFc Tag)

Catalog No. ABIN7274156

Recombinant TNFSF8 protein expressed in HEK-293 Cells.

Quick Overview for TNFSF8 Protein (AA 63-234) (mFc Tag) (ABIN7274156)

Target: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 63-234

Purification tag / Conjugate: This TNFSF8 protein is labelled with mFc Tag.

Purpose: Human CD30 Ligand/TNFSF8 Protein

Sequence: Gln63-Asp234

Characteristics: Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-Terminus.It contains Gln63-Asp234.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD30, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test).,Human CD30 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD30 Ligand is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Alternative Name: CD30 Ligand

Background: CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.

Molecular Weight: 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Bis-Tris PAGE result.

UniProt: P32971