CD33 Protein (CD33) (AA 18-259) (His-Avi Tag)

Catalog No. ABIN7274159

Recombinant CD33 protein expressed in HEK-293 Cells.

Quick Overview for CD33 Protein (CD33) (AA 18-259) (His-Avi Tag) (ABIN7274159)

Target: CD33

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 18-259

Purification tag / Conjugate: This CD33 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human Siglec-3/CD33 Protein

Sequence: Asp18-His259

Characteristics: Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus., It contains Asp18-His259.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Siglec-3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA.,Human Siglec-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Siglec-3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 20 mM MES, 500 mM NaCl, 200 mM L-Arginine ( pH 5.0). Normally 8 % mannitol is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD33

Alternative Name: Siglec-3

Background: CD33 (siglec-3), a well-known target in leukemia therapy, is an inhibitory sialoadhesin expressed in human leukocytes of the myeloid lineage and some lymphoid subsets, including NK cells. CD33 is the smallest siglec member. It preferentially binds to α2-6- and α2-3-sialylated glycans and strongly binds to sialylated ligands on leukemic cell lines.

Molecular Weight: 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.

UniProt: P20138