CD55 Protein (AA 35-353) (His tag)

Catalog No. ABIN7274193

This Recombinant CD55 protein is produced in HEK-293 Cells.

Quick Overview for CD55 Protein (AA 35-353) (His tag) (ABIN7274193)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 35-353

Purification tag / Conjugate: This CD55 protein is labelled with His tag.

Purpose: Human CD55 Protein

Sequence: Asp35-Ser353

Characteristics: Recombinant Human CD55 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp35-Ser353.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD55, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA (QC Test).,Human CD55 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD55 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55

Background: Decay Accelerating Factor (or CD55) is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection.

Molecular Weight: 35.8 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Bis-Tris PAGE result.

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity