CD55 Protein (AA 35-353) (His tag)
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- Target See all CD55 Proteins
- CD55 (Complement Decay-Accelerating Factor (CD55))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 35-353
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD55 protein is labelled with His tag.
- Purpose
- Human CD55 Protein
- Sequence
- Asp35-Ser353
- Characteristics
- Recombinant Human CD55 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp35-Ser353.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD55, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
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Complement Decay-Accelerating Factor (CD55) (AA 36-350) protein (His tag)
CD55 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Complement Decay-Accelerating Factor (CD55) (AA 35-362) protein (His tag)CD55 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Complement Decay-Accelerating Factor (CD55) (AA 1-381) protein (GST tag)CD55 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Complement Decay-Accelerating Factor (CD55) (AA 35-134) protein (GST tag)CD55 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Complement Decay-Accelerating Factor (CD55) protein (Fc Tag)CD55 Origin: Rat Host: HEK-293 Cells Recombinant > 82 % as determined by SDS-PAGE
Complement Decay-Accelerating Factor (CD55) (AA 35-353) protein (His tag)Crystallography grade CD55 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Complement Decay-Accelerating Factor (CD55) (AA 35-362) protein (His tag)Crystallography grade CD55 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD55 (Complement Decay-Accelerating Factor (CD55))
- Alternative Name
- CD55 (CD55 Products)
- Synonyms
- CR Protein, CROM Protein, DAF Protein, TC Protein, Daf Protein, Daf-GPI Protein, Daf1 Protein, GPI-DAF Protein, CD55 molecule (Cromer blood group) Protein, CD55 molecule, decay accelerating factor for complement Protein, CD55 Protein, Cd55 Protein
- Background
- Decay Accelerating Factor (or CD55) is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection.
- Molecular Weight
- 35.8 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result.
- Pathways
- Complement System, Regulation of Leukocyte Mediated Immunity
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