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CD5 Protein (CD5) (AA 25-371) (hFc-Avi Tag,Biotin)

This Recombinant CD5 protein is produced in HEK-293 Cells.
Catalog No. ABIN7274202
$715.42
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Quick Overview for CD5 Protein (CD5) (AA 25-371) (hFc-Avi Tag,Biotin) (ABIN7274202)

Target

See all CD5 Proteins
CD5

Protein Type

Recombinant

Biological Activity

Active

Origin

  • 19
  • 5
  • 5
  • 2
  • 1
Human

Source

  • 18
  • 6
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
HEK-293 Cells

Purity

> 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC
  • Protein Characteristics

    AA 25-371

    Purification tag / Conjugate

    This CD5 protein is labelled with hFc-Avi Tag,Biotin.

    Purpose

    Biotinylated Human CD5 Protein

    Sequence

    Arg25-Asn371

    Characteristics

    Recombinant Biotinylated Human CD5 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Arg25-Asn371.

    Sterility

    0.22 μm filtered

    Endotoxin Level

    Less than 1 EU per μg by the LAL method.

    Biological Activity Comment

    Immobilized Anti-CD5 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test).,Biotinylated Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human CD5 is greater than 95% as determined by SEC-HPLC.
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  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

    Buffer

    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

    Storage

    -20 °C,-80 °C

    Storage Comment

    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    CD5

    Alternative Name

    CD5

    Background

    CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion, expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.

    Molecular Weight

    67.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

    UniProt

    P06127
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