CD5 Protein (CD5) (AA 25-371) (Fc-Avi Tag,Biotin)
Quick Overview for CD5 Protein (CD5) (AA 25-371) (Fc-Avi Tag,Biotin) (ABIN7274202)
Target
See all CD5 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 25-371
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Purification tag / Conjugate
- This CD5 protein is labelled with Fc-Avi Tag,Biotin.
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Purpose
- Biotinylated Human CD5 Protein
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Sequence
- Arg25-Asn371
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Characteristics
- Recombinant Biotinylated Human CD5 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Arg25-Asn371.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Anti-CD5 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 16.0ng/ml determined by ELISA. See testing image for detail.
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CD5 (CD5) (AA 25-375) protein (His-Avi Tag)
CD5 Origin: Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD5 (CD5) (AA 25-371) protein (Fc-Avi Tag)CD5 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD5 (CD5) (AA 25-371) protein (His tag)CD5 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD5 (CD5) (AA 25-375) protein (His-Avi Tag,Biotin)CD5 Origin: Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD5 (CD5) (AA 25-371) protein (His tag)CD5 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD5 (CD5) (AA 24-370) protein (His tag)CD5 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
CD5 (CD5) (AA 25-372) protein (His tag)CD5 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD5 (CD5) (AA 1-495) protein (GST tag)CD5 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
CD5 (CD5) (AA 25-372) protein (His tag)CD5 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS
CD5 (CD5) (AA 1-371) protein (His tag)CD5 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD5
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Alternative Name
- CD5
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Background
- CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion, expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.
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Molecular Weight
- 67.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
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UniProt
- P06127
Target
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