CD47 Protein (CD47) (AA 19-139) (Biotin,His-Avi Tag)

Catalog No. ABIN7274233

This Recombinant CD47 protein is expressed in HEK-293 Cells.

Quick Overview for CD47 Protein (CD47) (AA 19-139) (Biotin,His-Avi Tag) (ABIN7274233)

Target: CD47

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-139

Purification tag / Conjugate: This CD47 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human CD47 Protein

Sequence: GIn19-Pro139

Characteristics: Recombinant Biotinylated Human CD47 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains GIn19-Pro139.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human CD47, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.,Biotinylated Human CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human CD47 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD47

Alternative Name: CD47

Background: CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.

Molecular Weight: 18.38 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.

UniProt: Q08722