CD79b Protein (AA 29-159) (His-Avi Tag,Biotin)

Catalog No. ABIN7274235

This Recombinant CD79b protein is produced in HEK-293 Cells.

Quick Overview for CD79b Protein (AA 29-159) (His-Avi Tag,Biotin) (ABIN7274235)

Target: CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 29-159

Purification tag / Conjugate: This CD79b protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human CD79B Protein

Sequence: Ala29-Asp159

Characteristics: Recombinant Biotinylated Human CD79B Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala29-Asp159.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-CD79B Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD79B, His Tag with the EC50 of 23.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))

Alternative Name: CD79B

Background: CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta-chain) is a 36-40 kDa member of the Ig-Superfamily. It is required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.

Molecular Weight: 18.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

UniProt: P40259-1

Pathways: BCR Signaling