CD28 Protein (CD28) (AA 19-152) (His-Avi Tag)

Catalog No. ABIN7274255

Recombinant CD28 protein expressed in HEK-293 Cells.

Quick Overview for CD28 Protein (CD28) (AA 19-152) (His-Avi Tag) (ABIN7274255)

Target: CD28

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Cynomolgus, Rhesus Monkey

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 19-152

Purification tag / Conjugate: This CD28 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein

Sequence: Asn19-Pro152

Characteristics: Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 43.61 uM as determined in SPR assay (Biacore T200).,Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15 nM as determined in a SPR assay (Biacore T200).,Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 23.9 ng/ml determined by ELISA (QC Test).,Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD28

Alternative Name: CD28

Background: CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Molecular Weight: 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Bis-Tris PAGE result.

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response