CD28 Protein (CD28) (AA 19-152) (Fc Tag,AVI tag,Biotin)

Catalog No. ABIN3137669

Product Details

Target: CD28

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 19-152

Origin: Human, Cynomolgus, Rhesus Monkey

Source: HEK-293 Cells

Purification tag / Conjugate: This CD28 protein is labelled with Fc Tag,AVI tag,Biotin.

Brand: MABSol®,PrecisionAvi

Sequence: AA 19-152

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 43.6 kDa. As a result of glycosylation, the protein migrates as 55-66 kDa under reducing (R) condition, and 110-130 kDa under non-reducing (NR) condition (SDS-PAGE).

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

References

Ganesan, Moon, Barakat: "Revealing the atomistic details behind the binding of B7-1 to CD28 and CTLA-4: A comprehensive protein-protein modelling study." in: Biochimica et biophysica acta. General subjects, Vol. 1862, Issue 12, pp. 2764-2778, (2019) (PubMed).

Target Details

Target: CD28

Alternative Name: CD28 (CD28 Products)

Synonyms: Tp44 Protein, CD28RNA Protein, cd28 Protein, CD28 molecule Protein, CD28 antigen Protein, Cd28 molecule Protein, CD28 molecule L homeolog Protein, CD28 Protein, Cd28 Protein, cd28.L Protein

Background: T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Molecular Weight: 43.6 kDa

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response