CD28 Protein (CD28) (AA 19-152) (Biotin,His-Avi Tag)
Quick Overview for CD28 Protein (CD28) (AA 19-152) (Biotin,His-Avi Tag) (ABIN7274256)
Target
See all CD28 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 19-152
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Purification tag / Conjugate
- This CD28 protein is labelled with Biotin,His-Avi Tag.
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Purpose
- Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein
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Sequence
- Asn19-Pro152
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Characteristics
- Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 41.59 uM as determined in SPR assay (Biacore T200).,Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA.,Biotinylated Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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CD28 (CD28) (AA 19-152) (Active) protein (His-Avi Tag)
CD28 Origin: Human, Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
CD28 (CD28) (AA 19-152) protein (His tag,mFc Tag)CD28 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
CD28 (CD28) (AA 19-152) (Active) protein (hFc-DYKDDDDK Tag)CD28 Origin: Human, Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD28 (CD28) (AA 19-152) (Active) protein (Fc Tag)CD28 Origin: Human, Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD28 (CD28) (AA 19-152) (Active) protein (Fc Tag,AVI tag,Biotin)CD28 Origin: Human, Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD28 (CD28) (Active) protein (His tag)CD28 Origin: Human, Cynomolgus Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
CD28 (CD28) (AA 19-152) (Active) protein (mFc Tag)CD28 Origin: Human, Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD28 (CD28) (AA 18-153) (Active) protein (His tag)CD28 Origin: Dog Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD28 (CD28) (AA 18-153) (Active) protein (mFc Tag)CD28 Origin: Dog Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD28 (CD28) (Active) protein (Biotin)CD28 Origin: Human, Cynomolgus Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD28
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Alternative Name
- CD28
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Background
- CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
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Molecular Weight
- 18.2 kDa. Due to glycosylation, the protein migrates to 38-55 kDa based on Bis-Tris PAGE result.
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UniProt
- P10747
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Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Target
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