CDCP1 Protein (AA 30-666) (Biotin,His-Avi Tag)

Catalog No. ABIN7274295

This Recombinant CDCP1 protein is produced in HEK-293 Cells.

Quick Overview for CDCP1 Protein (AA 30-666) (Biotin,His-Avi Tag) (ABIN7274295)

Target: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 30-666

Purification tag / Conjugate: This CDCP1 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human CDCP1 Protein

Sequence: Phe30-Leu666

Characteristics: Recombinant Biotinylated Human CDCP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe30-Leu666.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human CDCP1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA.,Biotinylated Human CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-CDCP1 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Biotinylated Human CDCP1, His-Avi Tag with an affinity constant of 3.97 nM as determined in BLI assay (Gator® Prime).,The purity of Biotinylated Human CDCP1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 100 mM Arginine, 8 % trehalose ( pH 7.4).

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Alternative Name: CDCP1

Background: Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.

Molecular Weight: 74.6 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result.

UniProt: Q9H5V8