CD40 Ligand Protein (CD40LG) (AA 113-261, Trimer) (His-DYKDDDDK Tag)

Catalog No. ABIN7274318

Recombinant CD40 Ligand protein expressed in HEK-293 Cells.

Quick Overview for CD40 Ligand Protein (CD40LG) (AA 113-261, Trimer) (His-DYKDDDDK Tag) (ABIN7274318)

Target: CD40 Ligand (CD40LG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Rhesus Monkey, Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 113-261, Trimer

Purification tag / Conjugate: This CD40 Ligand protein is labelled with His-DYKDDDDK Tag.

Purpose: Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein

Sequence: Met113-Leu261

Characteristics: Recombinant Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Met113-Leu261 trimer design.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 101.8ng/ml determined by ELISA.,Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human CD40, hFc Tag on ProA-Biosensor can bind Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), His-Flag Tag with an affinity constant of 0.23 μM as determined in BLI assay (Gator® Prime).,The purity of Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40 Ligand

Background: CD40 ligand or CD40L, also called CD154,  is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.

Molecular Weight: 52.4 kDa, Due to glycosylation, the protein migrates to 55 kDa based on Bis-Tris PAGE result.

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints