SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 16-1213, B.1.1.529 - Omicron, Trimer) (His tag)

Catalog No. ABIN7274386

This Recombinant SARS-CoV-2 Spike protein is expressed in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 16-1213, B.1.1.529 - Omicron, Trimer) (His tag) (ABIN7274386)

Target: SARS-CoV-2 Spike (SARS-CoV-2 S)

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 16-1213, B.1.1.529 - Omicron, Trimer

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with His tag.

Purpose: SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein

Sequence: Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F)

Characteristics: Recombinant SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F) trimer design. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 22.9ng/ml determined by ELISA.,SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human ACE2, hFc Tag on ProA-Biosensor can bind SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag with an affinity constant of 3.08 nM as determined in BLI assay (Gator® Prime).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike (SARS-CoV-2 S)

Alternative Name: SARS-COV-2 Spike

Target Type: Viral Protein

Background: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 136.70 kDa. Due to glycosylation, the protein migrates to 140-180 kDa based on Bis-Tris PAGE result.