SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 16-1188, D614G, Trimer) (His-Avi Tag)

Catalog No. ABIN7274402

Recombinant SARS-CoV-2 Spike protein expressed in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 16-1188, D614G, Trimer) (His-Avi Tag) (ABIN7274402)

Target: SARS-CoV-2 Spike (SARS-CoV-2 S)

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 16-1188, D614G, Trimer

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated SARS-COV-2 Spike S Trimer (D614G) Protein

Sequence: Val16-Glu1188(D614G)

Characteristics: Recombinant SARS-COV-2 Spike S Trimer(D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val16-Glu1188 (D614G) trimer design.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized SARS-COV-2 Spike S Trimer (D614G), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.,SARS-COV-2 Spike S Trimer (D614G) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of SARS-COV-2 Spike S Trimer (D614G) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike (SARS-CoV-2 S)

Alternative Name: SARS-COV-2 Spike

Target Type: Viral Protein

Background: The SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide, reaching near fixation in a matter of months. Recently, that D614G was been found more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.

Molecular Weight: 136.7 kDa. Due to glycosylation, the protein migrates to 175-230 kDa based on Bis-Tris PAGE result.