CTLA4 Protein (His-Avi Tag,Biotin)
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- Target See all CTLA4 Proteins
- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CTLA4 protein is labelled with His-Avi Tag,Biotin.
- Sequence
- Lys36-Asp161
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Biotinylated Human CTLA-4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product CTLA4 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
- Alternative Name
- CTLA-4 (CTLA4 Products)
- Synonyms
- CTLA-4 Protein, CTLA4 Protein, CD152 Protein, Cd152 Protein, Ctla-4 Protein, Ly-56 Protein, CD Protein, CELIAC3 Protein, GRD4 Protein, GSE Protein, IDDM12 Protein, sCTLA4 Protein, cytotoxic T-lymphocyte associated protein 4 Protein, cytotoxic T-lymphocyte-associated protein 4 Protein, CTLA4 Protein, Ctla4 Protein
- Background
- CTLA4, CD152, ICOS, CELIAC3, GRD4, GSE, ALPS5, IDDM12,CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation - a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.
- Molecular Weight
- 16.3 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
- UniProt
- P16410
- Pathways
- Cancer Immune Checkpoints
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