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CD226 Protein (CD226) (AA 19-247) (His-Avi Tag)

This Recombinant CD226 protein is expressed in HEK-293 Cells.
Catalog No. ABIN7274455

Quick Overview for CD226 Protein (CD226) (AA 19-247) (His-Avi Tag) (ABIN7274455)

Target

See all CD226 Proteins
CD226

Protein Type

Recombinant

Origin

  • 18
  • 5
  • 2
  • 1
  • 1
Human

Source

  • 16
  • 5
  • 3
  • 2
  • 1
HEK-293 Cells

Purity

> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
  • Protein Characteristics

    AA 19-247

    Purification tag / Conjugate

    This CD226 protein is labelled with His-Avi Tag.

    Purpose

    Human DNAM-1/CD226 Protein

    Sequence

    Glu19-Asn247

    Characteristics

    Recombinant Human DNAM-1/CD226 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu19-Asn247.

    Sterility

    0.22 μm filtered

    Endotoxin Level

    Less than 1EU per μg by the LAL method.

    Biological Activity Comment

    The affinity constant of 0.22μM as determined in SPR assay (Biacore T200). See testing image for detail.
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  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

    Buffer

    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

    Storage

    -20 °C,-80 °C

    Storage Comment

    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    CD226

    Alternative Name

    DNAM-1

    Background

    DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

    Molecular Weight

    28.9 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.

    UniProt

    Q15762

    Pathways

    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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