CD226 Protein (CD226) (AA 19-247) (Fc Tag)
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- Target See all CD226 Proteins
- CD226
- Protein Type
- Recombinant
- Protein Characteristics
- AA 19-247
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD226 protein is labelled with Fc Tag.
- Purpose
- Human DNAM-1/CD226 Protein
- Sequence
- Glu19-Asn247
- Characteristics
- Recombinant Human DNAM-1/CD226 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu19-Asn247.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Biotinylated Human CD155, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 4.8μg/ml determined by ELISA. The affinity constant of 0.234 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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CD226 (CD226) (AA 19-247) protein (His-Avi Tag)
CD226 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
CD226 (CD226) (AA 19-252) protein (His tag)CD226 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD226 (CD226) (AA 19-247) protein (His-Avi Tag,Biotin)CD226 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
CD226 (CD226) protein (mFc-His Tag)CD226 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD226 (CD226) (AA 19-252) (Active) protein (His tag)CD226 Origin: Cynomolgus Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD226 (CD226) protein (His tag)CD226 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
CD226 (CD226) (AA 21-127) protein (GST tag)CD226 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
CD226 (CD226) (Active) protein (His tag)CD226 Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
CD226 (CD226) (Active) protein (Fc Tag)CD226 Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
CD226 (CD226) (AA 19-254) protein (His tag)Crystallography grade CD226 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD226
- Alternative Name
- DNAM-1 (CD226 Products)
- Synonyms
- CD226 Protein, cd226 Protein, zgc:158817 Protein, BC051526 Protein, DNAM-1 Protein, DNAM1 Protein, Pta1 Protein, TLiSA1 Protein, PTA1 Protein, CD226 molecule Protein, CD226 antigen Protein, CD226 Protein, cd226 Protein, Cd226 Protein
- Background
- DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
- Molecular Weight
- 52.8 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result.
- UniProt
- Q15762
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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