DDR1 Protein (AA 21-417) (His tag)

Catalog No. ABIN7274460

Product Details

Target: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Protein Type: Recombinant

Protein Characteristics: AA 21-417

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This DDR1 protein is labelled with His tag.

Purpose: Human DDR1 Protein

Sequence: Asp21-Ala417

Characteristics: Recombinant Human DDR1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp21-Ala417.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human DDR1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA. The affinity constant of 0.806 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Alternative Name: DDR1 (DDR1 Products)

Background: Discoidin domain receptor1 (DDR1) is a collagen activated receptor tyrosine kinase and an attractive anti-fibrotic target. Its expression is mainly limited to epithelial cells located in several organs including skin, kidney, liver and lung. DDR1's biology is elusive, with unknown downstream activation pathways, however, it may act as a mediator of the stromal-epithelial interaction, potentially controlling the activation state of the resident quiescent fibroblasts.

Molecular Weight: 45.1 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Tris-Bis PAGE result.

Pathways: RTK Signaling, Smooth Muscle Cell Migration