DLL3 Protein (AA 27-477) (His tag)
Quick Overview for DLL3 Protein (AA 27-477) (His tag) (ABIN7274480)
Target
See all DLL3 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 27-477
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Purification tag / Conjugate
- This DLL3 protein is labelled with His tag.
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Purpose
- Cynomolgus DLL3 Protein
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Sequence
- Ala27-Ala477
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Characteristics
- Recombinant Cynomolgus DLL3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala27-Ala477.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Cynomolgus DLL3, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl, 200 mM L-arginine ( pH 8.5). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- DLL3 (delta Like Protein 3 (DLL3))
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Alternative Name
- DLL3
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Background
- Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).
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Molecular Weight
- 48.80 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.
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UniProt
- A0A2K5WSR1
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Pathways
- Notch Signaling
Target
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