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DLL3 Protein (AA 27-477) (His tag)

Recombinant DLL3 protein expressed in HEK-293 Cells.
Catalog No. ABIN7274480
$409.17
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Quick Overview for DLL3 Protein (AA 27-477) (His tag) (ABIN7274480)

Target

See all DLL3 Proteins
DLL3 (delta Like Protein 3 (DLL3))

Protein Type

Recombinant

Origin

  • 31
  • 8
  • 3
  • 2
  • 1
Cynomolgus

Source

  • 40
  • 3
  • 2
HEK-293 Cells

Purity

> 95 % as determined by Tris-Bis PAGE
  • Protein Characteristics

    AA 27-477

    Purification tag / Conjugate

    This DLL3 protein is labelled with His tag.

    Purpose

    Cynomolgus DLL3 Protein

    Sequence

    Ala27-Ala477

    Characteristics

    Recombinant Cynomolgus DLL3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala27-Ala477.

    Sterility

    0.22 μm filtered

    Endotoxin Level

    Less than 1EU per μg by the LAL method.

    Biological Activity Comment

    Immobilized Cynomolgus DLL3, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail.
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  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

    Buffer

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl, 200 mM L-arginine ( pH 8.5). Normally 8 % trehalose is added as protectant before lyophilization.

    Storage

    -20 °C,-80 °C

    Storage Comment

    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    DLL3 (delta Like Protein 3 (DLL3))

    Alternative Name

    DLL3

    Background

    Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).

    Molecular Weight

    48.80 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.

    UniProt

    A0A2K5WSR1

    Pathways

    Notch Signaling
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