DLL4 Protein (AA 27-524) (His-Avi Tag,Biotin)

Catalog No. ABIN7274487

This Recombinant DLL4 protein is produced in HEK-293 Cells.

Quick Overview for DLL4 Protein (AA 27-524) (His-Avi Tag,Biotin) (ABIN7274487)

Target: DLL4 (delta-Like 4 (DLL4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-524

Purification tag / Conjugate: This DLL4 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human DLL4 Protein

Sequence: Ser27-Pro524

Characteristics: Recombinant Biotinylated Human DLL4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser27-Pro524.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-DLL4 Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human DLL4, His Tag with the EC50 of 93.6ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: DLL4 (delta-Like 4 (DLL4))

Alternative Name: DLL4

Background: Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis, negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.

Molecular Weight: 55.7 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.

UniProt: Q9NR61

Pathways: Notch Signaling