TNFRSF10B Protein (AA 56-182) (hFc Tag)

Catalog No. ABIN7274496

This Recombinant TNFRSF10B protein is produced in HEK-293 Cells.

Quick Overview for TNFRSF10B Protein (AA 56-182) (hFc Tag) (ABIN7274496)

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 56-182

Purification tag / Conjugate: This TNFRSF10B protein is labelled with hFc Tag.

Purpose: Human TRAIL R2/DR5/TNFRSF10B Protein

Sequence: Ile56-Glu182

Characteristics: Recombinant Human TRAIL R2/DR5/TNFRSF10B Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ile56-Glu182.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Immobilized Human Trail, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R2, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA (QC Test).,Human TRAIL R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human TRAIL R2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Tris, 100 mM NaCl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Alternative Name: TRAIL R2

Background: DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.

Molecular Weight: 41.1 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.

UniProt: O14763

Pathways: p53 Signaling, Apoptosis, Positive Regulation of Endopeptidase Activity